Thursday, January 19, 2017

Candy Electrophoresis Lab

When we analyzed the results of our gel we found a few oddities in the results. First, the blue reference dye moved the shortest distance and the band was the largest of the colors. In addition, the purple dye was a different color than any of the reference dyes but traveled about the same distance as most of the dyes. Furthermore, the yellow dye for both the reference dye and the candy dye traveled the furthest, farther than any other color dye. The structure of dye that would most likely migrate similarly to the dyes that we examined would probably be Fast Green FCF because one side of the structure is negatively charged so it will move toward the positively charged cathode. Many dog food manufacturers put food coloring in dog food to make dog food look more appealing to dog owners, but this can cause problems with the dog as food coloring can contain bad chemicals.

In our daily lives, there are many foods that have artificial food colorings which can be bad for your health. For example, hot Cheetos, instant noodles, candy, and even cereal can contain artificial food colorings to make the food seem more appealing but at the same time more unhealthy. While we were doing our experiments there were two factors that could control the distance that the colored dye solutions migrated: was the amount of colorants in the dye and the size of the dye. The force that helps move the dyes through the gels is the electric current that was run through the electrophoresis gel.

On the other hand, the component that helped separate the food were the fibers in the electrophoresis gel as it would be harder for the larger molecules to travel through the gel. For example, if there were several DNA molecules with the molecular weights of 600, 1000, 2000, and 5000 daltons, the heaviest, or the 5000 dalton molecule, would move the shortest, and the smallest, or the 600 dalton molecule, would move the farthest.

No comments:

Post a Comment